NASA 1998 SBIR Phase I


PROPOSAL NUMBER: 98-1 08.01-9450

PROJECT TITLE: Iridium Oxide Biosensor Arrays for Multianalyte Bioprocess Monitoring

TECHNICAL ABSTRACT (LIMIT 200 WORDS)

NASA will require highly automated and low power monitoring and control to support long-term bioprocessing in the space environment The proposed innovation entails microfabricated sensors based on hydrous Ir oxide anodically formed on Ir thin film microelectrodes. These films can be used both as high surface area amperometric electrodes and as pH electrodes. We have shown further that these oxides can be grown to encapsulate and protect a range of enzymes and probably other large biomolecules (antibodies, DNA strands, co-factors, etc.). Using this approach, amperometric and potentiometric biosensors can be prepared simply and economically on microfabricated thin film Ir arrays. Multifunctional electrochemical biosensors can be made "on a chip" for monitoring O2, CO2, H2O2 and a wide range of enzyme substrates, including glucose, lactate, urea and amino acids. The goal of Phase I is to demonstrate a long-lived microfabricated Ir oxide electrochemical sensor/biosensor array for monitoring a group of analytes typical to cell culture production in a typical culture medium. Phase II will integrate this array into a micro-total analytical system for noninvasive monitoring and control of a HARV or STLV bioreactor, and/or a NASA in-house system.

POTENTIAL COMMERCIAL APPLICATIONS

Besides their use in space-based bioreactor systems, they are expected to impact the multibillion dollar bioprocessing industry for monitoring cell cultures, pharmaceutical production and fermentations.

NAME AND ADDRESS OF PRINCIPAL INVESTIGATOR

R. David Rauh
EIC Laboratories, Inc.
111 Downey Street
Norwood , MA 02062-2612

NAME AND ADDRESS OF OFFEROR

EIC Laboratories, Inc.
111 Downey Street
Norwood , MA 02062-2612