Form 9.B Project Summary

Chron: 972288

Proposal Number: 28.03-6941

Project Title: Detection and Quantitation of Rare DNA

Technical Abstract (Limit 200 words)

The study of evolutionary phenomena relies increasingly on the analysis of genomic DNA sequences, including fossil DNA. Because the samples to be studied are often available only in extremely small quantities, the capability to conduct analyses at very low copy number is essential. While PCR techniques have allowed detection down to a single copy, quantitation at very low copy number (less than a few hundred copies)has remained an unrealized goal.

BioTraces proposes the development of a quantitative direct detection of DNA method based on the ultrasensitive Multi Photon Detection (MPD) technique. MPD allows the detection and measurement of sub-zeptomole quantities of certain radioisotopes, including Iodine 125. Using a combination of MPD and BioTraces' proprietary SuperTracer technology, whereby several hundred radiolabels can be conjugated to a single DNA strand, detection of about ten copies and quantitation of a few tens of copies of DNA is expected.

Potential Commercial Applications (Limit 200 words)

The methods to be developed herein are generally applicable to DNA detection and quantitation for life science research and medical diagnostics. Quantitative direct (nonamplification) DNA detection assays at the level of 20-50 copies of DNA would be commercially important for detection of infectious diseases, cancer detection, and viral/bacterial load monitoring in connection with therapeutic treatment. The potential market for such assays is in excess of $1 billion per year.

Name and Address of Principal Investigator (Name,

Organization Name, Mail Address, City/State/Zip)

Andrzej K. Drukier

BioTraces, Inc.

10517-A West Drive

Fairfax , VA 22030

Name and Address of Offeror (Firm Name, Mail Address,

City/State/Zip)

E. James Wadiak

BioTraces, Inc.

10517-A West Drive

Fairfax , VA 22030