Project Title:

Glucose and 02 Sensors for Mammalian Cell Bioreactor Control

	  94-1	15.03	7770

	  Glucose and 02 Sensors for Mammalian Cell Bioreactor Control

Abstract:

Chemiluminescence (CL) sensors for D-glucose and 02 are proposed 
for bioprocess control. Mammalian cell bioreactors are perfused 
with fluid which provides nutrients required to sustain growth. 
Essential constituents of the perfusion fluid include glucose and 
oxygen. Glucose provides energy and serves as a carbon source 
through glycolytic and tricarboxylic acid oxidative metabolic 
pathways. Under oxygen limiting conditions, energy production is 
inefficient and accumulation of pyruvate and lactate tends to 
reduce pH below the physiological optimum. Control of glucose 
addition and tissue oxygenation is essential for efficient 
performance of the bioreactor. In the proposed innovation, 
glucose is detected by reaction with 02, catalyzed by immobilized 
glucose oxidase, producing H202 which is detected by CL. 02 is 
detected by a heterogeneously catalyzed reaction with iodide, 
forming HOI, which is detected by CL. In-line solid phase 
catalysts and solid state modules for pH adjustment and 
controlled release of reactants are used.

A strong potential exists for commercialization in the area of 
on-line continuous monitoring for glucose and oxygen for control 
of industrial fermentation processes which produce alcoholic 
beverages, vinegar, antibiotics, etc.

Key words

Umpqua Research Company
P. O. Box 791
Myrtle Creek, OR 97457