NASA SBIR 2008 Solicitation


PROPOSAL NUMBER: 08-1 X14.02-9864
SUBTOPIC TITLE: On Orbit Cell Counting and Analysis Capability
PROPOSAL TITLE: On Orbit Immuno-based, Label-free, White Blood Cell Counting System with MicroElectroMechanical Sensor (MEMS) Technology (OILWBCS-MEMS)

SMALL BUSINESS CONCERN (Firm Name, Mail Address, City/State/Zip, Phone)
Aurora Flight Sciences Corporation
9950 Wakeman Drive
Manassas, VA 20110 - 2702
(703) 369-3633

PRINCIPAL INVESTIGATOR/PROJECT MANAGER (Name, E-mail, Mail Address, City/State/Zip, Phone)
Dr. Liping Sun
1 Broadway, 12th Floor
Cambridge, MA 02142 - 1189
(617) 500-0279

Expected Technology Readiness Level (TRL) upon completion of contract: 3

TECHNICAL ABSTRACT (Limit 2000 characters, approximately 200 words)
Aurora Flight Sciences Corporation and our partner, Draper Laboratory, propose to develop an on orbit immuno-based, label-free, white blood cell counting system for simultaneous counting of peripheral blood cell subpopulations, including total white blood cells, the five white blood cell differential subgroups, and various lymphocyte subtypes, such as CD4 and CD8 positive cells, using Draper's MicroElectroMechanical Sensor (MEMS) based microfabricated arrayable Adhesive Stress Electrostatic Sensor (ASES) technology. The proposed ASES sensor uses a capacitance read-out method to electronically measure the sensor membrane displacement due to the surface stress caused by molecular binding, (e.g., antibody-antigen binding). Antibodies specific to the white blood cell surface protein markers (antigens) are precoated on the ASES sensor membrane to recognize the specific white blood cell types with inherently high specificity and sensitivity. Our proposed cell counting system can meet NASA's requirements for a microgravity compatible, miniaturized, light weight peripheral blood cell counting instrument capable of on-orbit cell counting, without high energy lasers, requiring minimal sample volume or exogenous (sheath) fluid to operate, and generating minimal biohazardous waste. This ASES blood cell counting system, once developed, can stand alone for white blood cell differential and subtype count, or become a complimentary instrument to others available on-orbit.

POTENTIAL NASA COMMERCIAL APPLICATIONS (Limit 1500 characters, approximately 150 words)
The proposed ASES white blood cell counting system could provide on orbit white blood cell counting capability, currently not available on orbit, for monitoring astronaut health and performing biomedical research on International Space Station (ISS), Crew Exploration Vehicles (CEV), and etc. With its high specificity/sensitivity, compact size, multiplexed counting capability, and automated operation, the proposed system can also provide a powerful cell counting capability for NASA's ground based biomedical research and development.

POTENTIAL NON-NASA COMMERCIAL APPLICATIONS (Limit 1500 characters, approximately 150 words)
Besides NASA's on orbit application, the proposed white blood cell counting system could be used to perform immuno-based cell counting similar to that done by flow cytometers. In general, any existing immuno-based cell counting performed for research and development, could potentially be accomplished with our proposed approach. The benefits of our proposed ASES cell counting system include high specificity and sensitivity for simultaneous counting of multiple cell types, compact size (the size of a cell phone), low power consumption, and full automation.

NASA's technology taxonomy has been developed by the SBIR-STTR program to disseminate awareness of proposed and awarded R/R&D in the agency. It is a listing of over 100 technologies, sorted into broad categories, of interest to NASA.

Biomedical and Life Support
Operations Concepts and Requirements

Form Generated on 11-24-08 11:56