NASA SBIR 00-1 SOLICITATION

FORM 9B - PROJECT SUMMARY


PROPOSAL NUMBER 00-1 11.01-7935 (Chron: 002066 )
PROJECT TITLE
Novel Biomatrix System for Human Tissue Growth & Angiogenesis in Microgavity



TECHNICAL ABSTRACT (LIMIT 200 WORDS)
One of NASA’s missions is to develop noninvasive models for monitoring the potentially deleterious effects of microgravity on human cell/tissue functions. Previous work has shown that gravitational changes can modify a variety of host cell-matrix interactions. Angiogenesis, an essential process for tissue growth and regeneration, is a coordinated process of activated endothelial cell proliferation and differentiation controlled by specific matrix and growth factor signals. However, little is known about the human angiogenesis process in microgravity, primarily due to the lack of defined functional models. We have created a new human biomatrix (Amgel)
culture system which supports endothelial cell growth and function. This unique biomatrix simulates human cell morphogenesis only in the presence of specific modulators (FGF, VEGF). We now propose to employ this natural matrix to further develop an all-human angiogenesis model adaptable for microgravity studies. Endothelial cell cultivation models with controllablebioactivity utilizing Amgel-layered disk and microbead configurations will be evaluated in rotary culture systems. New 3-D bioassays, generated with GF-enriched matrix, would allow examination of both early mitogenic and late angiogenic events. This R&D proposal should positively impact the fields of wound healing, tissue engineering and vascular disease.



POTENTIAL COMMERCIAL APPLICATIONS
No acceptable commercial model for examining human cell morphogenesis currently exists which utilizes a defined 3-D biomatrix system. We will develop, optimize and evaluate these microgravity adaptable human cell-matrix systems. Sale of proprietary Amgel and pre-packed bioassays would have a world-wide market in the hundreds of thousands of dollars. Thus, multiple utility of the Amgel model in biomedical and pharmaceutical research highlights its commercialization potential.



NAME AND ADDRESS OF PRINCIPAL INVESTIGATOR (Name, Organization Name, Mail Address, City/State/Zip)
Raj Singh, Ph.D.
Diversified Scientific Inc.
1601 12th Ave. South
Birmingham , AL 35205 - 4709



NAME AND ADDRESS OF OFFEROR (Firm Name, Mail Address, City/State/Zip)
Diversified Scientific Inc.
1601 12th Ave. South
Birmingham , AL 35205 - 4709